ARTIGOS CIENTÍFICOS PARASITOLOGIA. Diagnóstico sorológico de erliquiose canina com antígeno brasileiro de Ehrlichia canis. Serological diagnosis of. 5 jun. Introdução Erliquiose Canina Picada do carrapato Conclusão Argélia – Patogenia Hematologia – anemia arregenerativa Diagnóstico Uso. Erliquiose canina. 92 likes. A erliquiose canina é uma importante doença infecciosa transmitida pelo carrapato Rhipicephalus sanguineus (carrapato marrom).

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Epidemiological data collection The dog owners who participated in the study completed an epidemiological questionnaire, from which was acquired information about factors that could act as risk factors for infection, such as the presence of ticks, contact with other dogs, habitat, age, and gender. BMJ ; Blood samples were collected from dogs and submitted for indirect immunofluorescent assay and polymerase chain reaction testing for the detection of Ehrlichia spp.

This finding may be explained by the fact that diagnosis by IFA is more effective in the advanced stages of the disease, with reduced sensitivity in the initial phase of infection leading to false negatives. Of the dogs evaluated, 16 had positive results with all of the diagnostic techniques, and had negative results in all of them.

The negative and positive controls used for the dogs were canine sera on which serology had been performed in a prior study CARLOS et al. Comparison of a real-time PCR method with serology and blood smear analysis for diagnosis of human anaplasmosis: Canis familiares Ensaios e Cienc ; 12 2: The occurrence of R.

Ehrlichia canisisolation, DH82, immunofluorescence, serology. According to Azevedo et al. Ehrlichia Rev Bras Parasitol Vet ; 21 1: Dean AG, Arner T. These researchers collected samples from dogs treated at the Veterinary Hospital of the State University of Santa Cruz and in private veterinary clinics, as well as from domestic dogs through random home visits.


Veterinary Parasitologyv. In addition, the IFA can also generate cross-reactivity with other Anaplasmataceae agents.

Bioscience Journal

Survey of Ehrlichia canis antibodies among dogs in Israel. Sero-prevalence and risk indicators for canine ehrlichiosis in three rural areas of Brazil. Molecular detection of tick-borne bacterial agents in Brazilian and exotic captive carnivores. The absence erichiose E. The increasing interest of researchers in conducting studies about elrichiose disease is due to the high morbidity and mortality of canine ehrlichiosis in Brazil. Serologic prevalence of Dirofilaria immintis, Ehrlichia canis and Borrelia burgdorferi infection in Brazil.

To analyze the risk factors, all animals exposed erlichioze agent were considered seropositives on IFA or positives on blood-smear examination or nested-PCR testing. J Vet Diagn Investv. Molecular studies indicate that new strains of Ehrlichia spp. Rev Bras Parasitol Vet ; 18 4: Ehrlichia canisE.

Erliquiose canina

Thus, the objectives of this study were to determine the canine positivity for Ehrlichia spp. Therefore, the low frequency of positive results obtained with the blood smears in this study may be explained by the higher chance of false negatives. Younger dogs and rural habitat were protective factors and presence of ticks and contact with other dogs were the risk factors associated with exposure to the agent. On IFA, the seroprevalence The dogs positive in one of the three techniques were considered exposed Furthermore, this study identified factors associated with exposure to the agent in dogs in this locality.

Seroepidemiology of canine babesiosis and ehrlichiosis in a hospital population. J Mol Diagnv. For each house visited, a maximum of two dogs were evaluated.

Anaplasmataceae in dogs and Acari: Five milliliters were dispensed into plastic tubes with an anticoagulant EDTAand 3 mL was dispensed into tubes without an anticoagulant.

This bacterium mainly parasitizes the cells of the mononuclear phagocyte system and causes clinical and hematological abnormalities such as fever, anorexia, vomiting, diarrhea, petechial hemorrhages, anemia, and thrombocytopenia MOREIRA et al.


Serological diagnosis of tropical canine pancytopenia by indirect immunofluorescence.

However, this was not the focus of this research, which aimed to detect the DNA of only erlichiosw E. Risk factors and clinical disorders of canine ehrlichiosis in the South of Bahia, Brazil. Statistical analyses To analyze the risk factors, all animals exposed to agent were considered seropositives on IFA or positives on blood-smear examination or nested-PCR testing.

The samples included in the study by Moreira et al. Clin Vetn. Evaluation of neutrophil oxidative metabolism in canine monocytic ehrlichiosis. Vet Parasitolv. This may explain the high seropositivity of these animals.

Clinical and molecular evaluation of dogs with ehrlichiosis

Prevalence of Ehrlichia canis Rickettsiales: In addition to the above mentioned points, because serological testing identifies only antibodies, a positive case cabina serology may appear negative on PCR testing because of eradication of the Ehrlichia organisms by the immune system. Ehrlichia canis J Clin Microbiol ; 36 1: This may also explain the absence of detection of E.

Clinical and molecular evaluation of dogs with ehrlichiosis. Canine monocytic ehrlichiosis CME. On the IFA and nested-PCR test, 70 dogs were positive with the first diagnostic technique and negative with the second, whereas 43 dogs were positive with the second technique and negative with the first Figure 1. Dogs that had any contact with other dogs residents or not and those that were parasitized by the ticks of R.